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Host: Mouse
Target Protein: FOXO3A
Clonality: Monoclonal
Isotype: IgG2A/kappa
Entrez Gene: 2309
Swiss Prot: O43524
Source: KLH conjugated synthetic peptide derived from human FOXO3A
Purification: Purified by Protein G.
Storage Buffer: PBS, Glycerol, BSA.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: Lot Dependent
Applications:
WB(1:300-5000)
IHC-F(1:50-200)
IF(1:50-200)
IHC-P(1:50-200)
ICC/IF(1:50-200)
Predicted Molecular Weight: 74
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with FOXO3A monoclonal antibody, unconjugated (bsm-60740M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Prastate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FOXO3A Monoclonal Antibody, Unconjugated (bsm-60740M) at 1:100 overnight at 4°C, followed by conjugation to bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FOXO3A Monoclonal Antibody, Unconjugated (bsm-60740M) at 1:100 overnight at 4°C, followed by conjugation to bs-40296G-HRP and DAB (C-0010) staining.