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Host: Mouse
Target Protein: HDAC3
Clonality: Monoclonal
Isotype: IgG2b, Kappa
Entrez Gene: 8841
Swiss Prot: O15379
Source: KLH conjugated synthetic peptide derived from human HDAC3
Purification: Purified by Protein G.
Storage Buffer: PBS, Glycerol, BSA.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Predicted Molecular Weight: 47
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature.Primary Antibody (green):Mouse Anti-HDAC3 antibody (bsm-60742M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.