Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human A549 cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human 293T cell lysates; Lane 7: Human HepG2 cell lysates; Lane 8: Human Jurkat cell lysates probed with STIP1 Monoclonal Antibody, Unconjugated (bsm-60759R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with STIP1 | HOP Recombinant Monoclonal Antibody, Unconjugated (bsm-60759R) at 1:100 overnight at 4°C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with STIP1 | HOP Recombinant Monoclonal Antibody, Unconjugated (bsm-60759R) at 1:100 overnight at 4°C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with STIP1 | HOP Recombinant Monoclonal Antibody, Unconjugated (bsm-60759R) at 1:100 overnight at 4°C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with STIP1 | HOP Recombinant Monoclonal Antibody, Unconjugated (bsm-60759R) at 1:100 overnight at 4°C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with STIP1 | HOP Recombinant Monoclonal Antibody, Unconjugated (bsm-60759R) at 1:100 overnight at 4°C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with STIP1 | HOP Recombinant Monoclonal Antibody, Unconjugated (bsm-60759R) at 1:100 overnight at 4°C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with STIP1 | HOP Recombinant Monoclonal Antibody, Unconjugated (bsm-60759R) at 1:100 overnight at 4°C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with STIP1 | HOP Recombinant Monoclonal Antibody, Unconjugated (bsm-60759R) at 1:100 overnight at 4°C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Thyroid Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with STIP1 | HOP Recombinant Monoclonal Antibody, Unconjugated (bsm-60759R) at 1:100 overnight at 4°C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with STIP1 | HOP Recombinant Monoclonal Antibody, Unconjugated (bsm-60759R) at 1:100 overnight at 4°C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.


The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-STIP1 antibody (bsm-60759R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.