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Host: Rabbit
Target Protein: Nestin
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 10763
Swiss Prot: P48681
Source: KLH conjugated synthetic peptide derived from human Nestin
Purification: Purified by Protein A.
Storage Buffer: PBS, Glycerol, BSA.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
WB(WB(1:1000-5000))
IHC-P(IHC-P(1:200-1000))
IHC-F(IHC-F(1:200-1000))
IF(IHC-P)(IF(IHC-P)(1:200-1000))
IF(ICC)(IF(ICC)(1:100-500))
Predicted Molecular Weight: 178
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with Nestin monoclonal antibody, unconjugated (bsm-60783R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nestin Monoclonal Antibody, Unconjugated(bsm-60783R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nestin Monoclonal Antibody, Unconjugated(bsm-60783R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nestin Monoclonal Antibody, Unconjugated(bsm-60783R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nestin Monoclonal Antibody, Unconjugated(bsm-60783R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nestin Monoclonal Antibody, Unconjugated(bsm-60783R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nestin Monoclonal Antibody, Unconjugated(bsm-60783R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nestin Monoclonal Antibody, Unconjugated(bsm-60783R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
4% Paraformaldehyde-fixed U251 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Nestin) monoclonal Antibody, unconjugated (bsm-60783R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.