bsm-60788R [Primary Antibody]
SOX2 Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SOX2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6657

Swiss Prot: P48432

Source: KLH conjugated synthetic peptide derived from human SOX2

Purification: Purified by Protein A.

Storage Buffer: PBS, Glycerol, BSA

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Size: 100ul

Concentration: 1mg/ml

Applications: IHC-P(1:200-400)

Predicted Molecular Weight: 34


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SOX2) Monoclonal Antibody, Unconjugated (bsm-60788R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SOX2) Monoclonal Antibody, Unconjugated (bsm-60788R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat embryo); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SOX2) Monoclonal Antibody, Unconjugated (bsm-60788R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse embryo); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SOX2) Monoclonal Antibody, Unconjugated (bsm-60788R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.