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Host: Rabbit
Target Protein: ACTH
Clonality: Recombinant
Isotype: IgG
Source: KLH conjugated synthetic peptide derived from human ACTH
Purification: Purified by Protein A.
Storage Buffer: PBS, Glycerol, BSA.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
WB(1:1000-5000)
IHC-P(1:200-800)
IHC-F(1:200-800)
IF(1:200-800)
Predicted Molecular Weight: 4.5/29
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Human pituitary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ACTH Monoclonal Antibody, Unconjugated(bsm-60798R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ACTH Monoclonal Antibody, Unconjugated(bsm-60798R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with ACTH monoclonal antibody, unconjugated (bsm-60798R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.