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Host: Rabbit
Target Protein: SOD1
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 6647
Swiss Prot: P00441
Source: KLH conjugated synthetic peptide derived from human SOD1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
WB(1:1000-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Predicted Molecular Weight: 17
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species: Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SOD1) Monoclonal Antibody, Unconjugated (bsm-60838R) at 1:300 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SOD1) Monoclonal Antibody, Unconjugated (bsm-60838R) at 1:300 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SOD1) Monoclonal Antibody, Unconjugated (bsm-60838R) at 1:300 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SOD1) Monoclonal Antibody, Unconjugated (bsm-60838R) at 1:300 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SOD1) Monoclonal Antibody, Unconjugated (bsm-60838R) at 1:300 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Mouse Placenta tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Placenta tissue lysates; Lane 6: Human HepG2 cell lysates; Lane 7: Human HeLa cell lysates; Lane 8: Human Jurkat cell lysates; Lane 9: Human MCF-7 cell lysates probed with SOD1 Monoclonal Antibody, Unconjugated (bsm-60838R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.