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Host: Rabbit
Target Protein: CNN1
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 1264
Swiss Prot: P51911
Source: A synthesized peptide derived from human Calponin 1
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Conjugation: Biotin
Excitation/ Emission: N/A
Size: 100µL
Concentration: 1mg/ml
Applications:
WB(WB(1:2000-10000))
IHC-P(IHC-P(1:100-500))
IHC-F(IHC-F(1:100-500))
Predicted Molecular Weight: 33
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with CNN1 monoclonal antibody, Biotin conjugated (bsm-60845R-Bio) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CNN1 Monoclonal Antibody (bsm-60845R-Bio) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human fundus of stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CNN1 Monoclonal Antibody (bsm-60845R-Bio) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CNN1 Monoclonal Antibody (bsm-60845R-Bio) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CNN1 Monoclonal Antibody (bsm-60845R-Bio) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CNN1 Monoclonal Antibody (bsm-60845R-Bio) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CNN1 Monoclonal Antibody (bsm-60845R-Bio) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CNN1 Monoclonal Antibody (bsm-60845R-Bio) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CNN1 Monoclonal Antibody (bsm-60845R-Bio) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CNN1 Monoclonal Antibody (bsm-60845R-Bio) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CNN1 Monoclonal Antibody (bsm-60845R-Bio) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CNN1 Monoclonal Antibody (bsm-60845R-Bio) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CNN1 Monoclonal Antibody (bsm-60845R-Bio) at 1:200 overnight at 4°C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CNN1 Monoclonal Antibody (bsm-60845R-Bio) at 1:200 overnight at 4°C, and DAB (C-0010) staining.