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Host: Mouse
Target Protein: CHAT
Clonality: Monoclonal
Isotype: IgG1/kappa
Entrez Gene: 12647
Swiss Prot: Q03059
Source: KLH conjugated synthetic peptide derived from mouse ChAT
Purification: purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100µl
Concentration: Lot Dependent
Applications:
WB
IHC-F
IF(IHC-P)
IHC-P
Predicted Molecular Weight: 82
Cross Reactive Species:
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with CHAT monoclonal antibody, unconjugated (bsm-60892M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CHAT Monoclonal Antibody, Unconjugated(bsm-60892M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CHAT Monoclonal Antibody, Unconjugated(bsm-60892M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024)and DAB (C-0010) staining.