bsm-60911M [Primary Antibody]
TLR4 Monoclonal Antibody
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Host: Mouse

Target Protein: TLR4

Clonality: Monoclonal

Isotype: IgG1

Entrez Gene: 7099

Swiss Prot: O00206

Source: KLH conjugated synthetic peptide derived from human TLR4

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.

Size: 100µL

Concentration: 1ug/ul

Predicted Molecular Weight: 90


Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with TLR4 monoclonal antibody, unconjugated (bsm-60911M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.