bsm-60916M [Primary Antibody]
S100A4 Monoclonal Antibody
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Host: Mouse

Target Protein: S100A4

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6275

Swiss Prot: P26447

Source: KLH conjugated synthetic peptide derived from human S100A4

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Size: 100µl

Concentration: Lot Dependent

Applications: IHC-F
IF(IHC-P)
IHC-P

Predicted Molecular Weight: 12


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (bsm-60916M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (Human Cervical Cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (bsm-60916M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (Human Colon Cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (bsm-60916M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (Human Tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (bsm-60916M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (Human lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (bsm-60916M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (Human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (bsm-60916M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (Human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (bsm-60916M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (Human Gastric Cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (bsm-60916M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.