bsm-60931R [Primary Antibody]
ATF3 Monoclonal Antibody
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Host: Rabbit

Target Protein: ATF3

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 467

Swiss Prot: P18847

Source: KLH conjugated synthetic peptide derived from human ATF

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.

Size: 100µl

Concentration: Lot Dependent

Applications: WB
IHC-F
IF(IHC-P)
IHC-P

Predicted Molecular Weight: 20


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

RAW264.7 (M) cells were treated with or without LPS (1ug/ml) for 2 h, 25 μg total protein per lane of cell lysates (see on figure) probed with ATF3 monoclonal antibody, unconjugated (bsm-60931R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ATF3 Monoclonal Antibody, Unconjugated (bsm-60931R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.