bsm-60943R [Primary Antibody]
Phospho-RPS6 (Ser240 + Ser244) Recombinant Antibody
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Host: Rabbit

Target Protein: Phospho-RPS6 (Ser240 + Ser244)

Modification Site: Ser240 + Ser244

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 6194

Swiss Prot: P62753

Source: KLH conjugated Synthesised phosphopeptide derived from human RPS6 around the phosphorylation site of Ser240/244

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Size: 100µL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 27


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-RPS6 (Ser240 + Ser244) Monoclonal Antibody, Unconjugated (bsm-60943R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


MCF-7 (H) Serum starvation overnight, then grown in 20% FBS media for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-RPS6 (Ser240 + Ser244) monoclonal antibody, unconjugated (bsm-60943R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.