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Host: Rabbit
Target Protein: Mono-Methyl-Histone H3 (Lys27)
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 8350
Swiss Prot: Q71DI3
Source: KLH conjugated synthesised acetylpeptide derived from human Histone H3 around methylation site of mono methyl K27
Purification: purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: Lot Dependent
Applications: ChIP
For research use only. Not intended for diagnostic or therapeutic use.
Chromatin immunoprecipitation was performed using cross-linked chromatin from 5×10⁶ HeLa cells per IP, with 5 μg of bsm-60972R antibody or 5 μg of normal rabbit IgG(negative control bs-0295P) , and 36 μL of Protein A/G MagBeads per IP. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH-CDS, LDHA and FOXO3A-intron 1 regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.