support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
Host: Rabbit
Target Protein: Acetyl-Histone H3 (Lys27)
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 8350
Swiss Prot: Q71DI3
Source: A synthesized peptide derived from human Histone H3.1 around the acetylation site of K27
Purification: purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: Lot Dependent
Applications:
WB(1:500-2000)
IHC-P(1:50-200)
IHC-F(1:50-200)
ICC/IF(1:50-200)
ELISA(1:5000-10000)
ChIP(5μg/IP)
Predicted Molecular Weight: 15 kDa
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Chromatin immunoprecipitation was performed using cross-linked chromatin from 5×10⁶ HeLa cells (treated with 5 mM sodium butyrate for 24 hours) per IP, with 5 μg of bsm-60973R antibody or 5 μg of normal rabbit IgG(negative control bs-0295P) , and 20 μL of Protein A/G MagBeads per IP. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH intron 2,GAPDH intron 2, GAPDH promoter, RPL30 exon 3 and AFM intron 1. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.