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Host: Rabbit
Target Protein: Acetyl-Histone H3 (Lys9)
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 8968
Swiss Prot: Q71DI3
Source: KLH conjugated synthesised acetylpeptide derived from human Histone H3 around the acetylation site of K9
Purification: purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: Lot Dependent
Applications:
ELISA(1:500-1000)
IHC-F(1:50-200)
IF(1:50-200)
ChIP
IHC-P(1:50-200)
Predicted Molecular Weight: 15
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H3 (Lys9) Monoclonal Antibody, Unconjugated (bsm-60976R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H3 (Lys9) Monoclonal Antibody, Unconjugated (bsm-60976R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H3 (Lys9) Monoclonal Antibody, Unconjugated (bsm-60976R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Acetyl-Histone H3 (Lys9) Monoclonal Antibody, Unconjugated (bsm-60976R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Chromatin immunoprecipitation was performed using cross-linked chromatin from 5×10⁶ HeLa cells (treated with 5 mM sodium butyrate for 24 hours) per IP, with 5 μg of bsm-60976R antibody or 5 μg of normal rabbit IgG(negative control bs-0295P) , and 20 μL of Protein A/G MagBeads per IP. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS and RPL30 exon 3 regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.