bsm-61011R [Primary Antibody]
eIF2B epsilon Recombinant Antibody
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Host: Rabbit

Target Protein: eIF2B epsilon

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 8893

Swiss Prot: Q13144

Source: KLH conjugated synthetic peptide derived from human eIF2B epsilon

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq, Nov 2009]

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 80


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with eIF2B epsilon monoclonal antibody, unconjugated (bsm-61011R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF2B epsilon Monoclonal Antibody, Unconjugated(bsm-61011R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.