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Host: Rabbit
Target Protein: AMPK alpha (Thr172)
Clonality: Recombinant
Isotype: IgG
Source: A synthesized peptide derived from human AMPK alpha 1 around the phosphorylation site of T183
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µl
Concentration: Lot dependent
Applications: WB(WB=1:500-2000)
Predicted Molecular Weight: 64
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
293T (H) cells were treated with or without Oligomycin (5uM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-AMPK alpha (Thr172) monoclonal antibody, unconjugated (bsm-61057R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.