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Host: Rabbit
Target Protein: MEIS2
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 4212
Swiss Prot: O14770
Source: KLH conjugated synthetic peptide derived from human MEIS2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at 4°C for short term. Store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: Lot dependent
Applications: FCM(FCM(1ug/Test))
Predicted Molecular Weight: 52
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-MEIS2 antibody (bsm-61073R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 μg/test.Blank control (black): PBS. Acquisition of 20,000 events was performed.