bsm-61087R [Primary Antibody]
Galectin 3 Recombinant Antibody
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Host: Rabbit

Target Protein: Galectin 3

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 3958

Swiss Prot: P17931

Source: KLH conjugated synthetic peptide derived from human Galectin 3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

Size: 100µL

Concentration: Lot dependent

Applications: WB(WB(1:1000-5000))
IHC-P(IHC-P(1:200-800))
IHC-F(IHC-F(1:200-800))
IF(IHC-P)(IF(IHC-P)(1:200-800))

Predicted Molecular Weight: 29


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Galectin 3 monoclonal antibody, unconjugated (bsm-61087R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with Galectin 3 Monoclonal Antibody, Unconjugated (bsm-61087R) at 1:200 overnight at 4°C, followed by a BF594 conjugated Goat Anti-Rabbit IgG antibody (bs-0295G- BF594) for 90 minutes, and DAPI for nuclei staining.


Paraformaldehyde-fixed, paraffin embedded Human testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with Galectin 3 Monoclonal Antibody, Unconjugated (bsm-61087R) at 1:200 overnight at 4°C, followed by a BF594 conjugated Goat Anti-Rabbit IgG antibody (bs-0295G- BF594) for 90 minutes, and DAPI for nuclei staining.


Paraformaldehyde-fixed, paraffin embedded Human small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with Galectin 3 Monoclonal Antibody, Unconjugated (bsm-61087R) at 1:200 overnight at 4°C, followed by a BF594 conjugated Goat Anti-Rabbit IgG antibody (bs-0295G- BF594) for 90 minutes, and DAPI for nuclei staining.


Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with Galectin 3 Monoclonal Antibody, Unconjugated (bsm-61087R) at 1:200 overnight at 4°C, followed by a BF594 conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-BF594) for 90 minutes, and DAPI for nuclei staining.


Paraformaldehyde-fixed, paraffin embedded Human testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with Galectin 3 Monoclonal Antibody, Unconjugated (bsm-61087R) at 1:200 overnight at 4°C, followed by a BF594 conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-BF594) for 90 minutes, and DAPI for nuclei staining.


Paraformaldehyde-fixed, paraffin embedded Human small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with Galectin 3 Monoclonal Antibody, Unconjugated (bsm-61087R) at 1:200 overnight at 4°C, followed by a BF594 conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-BF594) for 90 minutes, and DAPI for nuclei staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated(bsm-61087R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated(bsm-61087R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated(bsm-61087R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated(bsm-61087R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated(bsm-61087R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated(bsm-61087R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated(bsm-61087R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated(bsm-61087R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Thyroid Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Galectin 3 Monoclonal Antibody, Unconjugated (bsm-61087R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Galectin 3 Monoclonal Antibody, Unconjugated (bsm-61087R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.