bsm-61100R [Primary Antibody]
RNF20 Recombinant Antibody
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Host: Rabbit

Target Protein: RNF20

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 56254

Swiss Prot: Q5VTR2

Source: KLH conjugated synthetic peptide derived from human RNF20

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation.

Size: 100µL

Concentration: Lot dependent

Applications: WB(1:500-2000)
IP(1:20-50)
Flow-Cyt(1ug/Test)
ICC/IF(1:50-100)

Predicted Molecular Weight: 114


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot analysis of (1) 293 cell lysate; (2) HeLa cell lysate. Using RNF20 (bsm-61100R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.