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Host: Rabbit
Target Protein: CCR9
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 10803
Swiss Prot: P51686
Source: KLH conjugated synthetic peptide derived from human CCR9
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100µL
Concentration: Lot dependent
Applications:
WB(WB(1:500-2000))
FCM(FCM(1ug/Test))
IF(ICC)(IF(ICC)(1:50-200))
Predicted Molecular Weight: 42
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with CCR9 monoclonal antibody, unconjugated (bsm-61106R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Molt-4 (H) cell; Antibody incubation with (CCR9) monoclonal Antibody, unconjugated (bsm-61106R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Molt-4(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-CCR9 antibody (bsm-61106R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.