bsm-61115R [Primary Antibody]
OLIG2 Recombinant Antibody
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Host: Rabbit

Target Protein: OLIG2

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 10215

Swiss Prot: Q13516

Source: KLH conjugated synthetic peptide derived from human Oligo2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Functions together with ZNF488 to promote oligodendrocyte differentiation. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.

Size: 100µL

Concentration: 1mg/ml

Applications: IHC-P(1:200-800)
IHC-F(1:200-800)
IF(1:200-800)
ICC/IF(1:100-200)

Predicted Molecular Weight: 32


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OLIG2 Monoclonal Antibody, Unconjugated(bsm-61115R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OLIG2 Monoclonal Antibody, Unconjugated(bsm-61115R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OLIG2 Monoclonal Antibody, Unconjugated(bsm-61115R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OLIG2 Monoclonal Antibody, Unconjugated(bsm-61115R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OLIG2 Monoclonal Antibody, Unconjugated(bsm-61115R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OLIG2 Monoclonal Antibody, Unconjugated (bsm-61115R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OLIG2 Monoclonal Antibody, Unconjugated (bsm-61115R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose pink, bs-0295G-BF647), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OLIG2 Monoclonal Antibody, Unconjugated (bsm-61115R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose pink, bs-0295G-BF647), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OLIG2 Monoclonal Antibody, Unconjugated (bsm-61115R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose pink, bs-0295G-BF647), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum. Merged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue) . DAPI (dark blue) was used as a nuclear counter stain.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum. Merged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue) . DAPI (dark blue) was used as a nuclear counter stain.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum. Merged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue) . DAPI (dark blue) was used as a nuclear counter stain.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The first round ,antibody incubation with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488). The second round , antibody incubation with Olig2 Monoclonal Antibody, Unconjugated (bsm-61115R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594). DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The first round ,antibody incubation with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488). The second round , antibody incubation with Olig2 Monoclonal Antibody, Unconjugated (bsm-61115R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594). DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The first round ,antibody incubation with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488). The second round , antibody incubation with Olig2 Monoclonal Antibody, Unconjugated (bsm-61115R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594). DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min. The first round ,antibody incubation with MBP Monoclonal Antibody, Unconjugated (bsm-33932M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488). The second round , antibody incubation with Olig2 Monoclonal Antibody, Unconjugated (bsm-61115R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (red, bs-0295G-BF594). DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OLIG2 Monoclonal Antibody, Unconjugated (bsm-61115R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.