bsm-61127R [Primary Antibody]
Complement C9 Recombinant Antibody
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Host: Rabbit

Target Protein: Complement C9

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 735

Swiss Prot: P02748

Source: KLH conjugated synthetic peptide derived from human Complement C9

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 63


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Complement C9 monoclonal antibody, unconjugated (bsm-61127R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Complement C9 Monoclonal Antibody, Unconjugated(bsm-61127R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.