support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
Host: Rabbit
Target Protein: CD11c
Clonality: Recombinant
Isotype: IgG1 κ
Entrez Gene: 3687
Swiss Prot: P20702
Source: KLH conjugated synthetic peptide derived from human CD11c
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100µL
Concentration: 1mg/ml
Applications:
WB(WB(1:500-2000))
FCM(FCM(1ug/Test))
IHC-P(IHC-P(1:200-800))
IHC-F(IHC-F(1:200-800))
IF(IHC-P)(IF(IHC-P)(1:200-800))
IF(ICC)(IF(ICC)(1:100-200))
IP(IP(1:20-50))
Predicted Molecular Weight: 128
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
THP-1 (H) cells were treated with or without PMA (50 ng/mL) for 72 h, 25 μg total protein per lane of cell lysates (see on figure) probed with CD11c monoclonal antibody, unconjugated (bsm-61135R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11c Monoclonal Antibody, Unconjugated(bsm-61135R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11c Monoclonal Antibody, Unconjugated(bsm-61135R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11c Monoclonal Antibody, Unconjugated(bsm-61135R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11c Monoclonal Antibody, Unconjugated(bsm-61135R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
The THP-1（treated with 100 ng/mL PMA for 24 h） (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.),followed by secondary antibody incubation for 40 min at room temperature.Primary Antibody (green):Rabbit Anti-CD11c antibody (bsm-61135R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.