bsm-61150R [Primary Antibody]
AKT1S1 Recombinant Antibody
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Host: Rabbit

Target Protein: AKT1S1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 84335

Swiss Prot: Q96B36

Source: KLH conjugated synthetic peptide derived from human AKT1S1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth.

Size: 100µL

Concentration: Lot dependent

Applications: WB(WB(1:500-2000))
IP(IP(1:20-50))

Predicted Molecular Weight: 27


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with AKT1S1 monoclonal antibody, unconjugated (bsm-61150R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


25 ug total protein per lane of various lysates (see on figure) probed with AKT1S1 monoclonal antibody, unconjugated (bsm-61150R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.