bsm-61196R [Primary Antibody]
Phospho-beta Arrestin 1 (Ser412) Recombinant Antibody
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Host: Rabbit

Target Protein: beta Arrestin 1

Modification Site: Ser412

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 408

Swiss Prot: P49407

Source: KLH conjugated synthetic peptide derived from human beta Arrestin 1 around the phosphorylation site of S412

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Functions in regulating agonist-mediated G-protein coupled receptor (GPCR) signaling by mediating both receptor desensitization and resensitization processes. During homologous desensitization, beta-arrestins bind to the GPRK-phosphorylated receptor and sterically preclude its coupling to the cognate G-protein; the binding appears to require additional receptor determinants exposed only in the active receptor conformation.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 47


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Phospho-beta Arrestin 1 (Ser412) monoclonal antibody, unconjugated (bsm-61196R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.