bsm-61216R [Primary Antibody]
PGK1 Recombinant Antibody
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Host: Rabbit

Target Protein: PGK1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 5230

Swiss Prot: P00558

Source: KLH conjugated synthetic peptide derived from human PGK1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein.

Size: 100µL

Concentration: Lot dependent

Applications: WB(WB(1:500-2000))
FCM(FCM(1:50-100))
IF(ICC)(IF(ICC)(1:50-200))

Predicted Molecular Weight: 45


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with PGK1 monoclonal antibody, unconjugated (bsm-61216R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.