bsm-61226R [Primary Antibody]
Apolipoprotein E Recombinant Antibody
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Host: Rabbit

Target Protein: Apolipoprotein E

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 348

Swiss Prot: P02649

Source: KLH conjugated synthetic peptide derived from human Apolipoprotein E

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 36


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human liver cancer;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Apolipoprotein E Monoclonal Antibody, Unconjugated (bsm-61226R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Apolipoprotein E Monoclonal Antibody, Unconjugated (bsm-61226R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Apolipoprotein E Monoclonal Antibody, Unconjugated (bsm-61226R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Apolipoprotein E Monoclonal Antibody, Unconjugated (bsm-61226R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human skin;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Apolipoprotein E Monoclonal Antibody, Unconjugated (bsm-61226R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen ;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Apolipoprotein E Monoclonal Antibody, Unconjugated (bsm-61226R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney ;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Apolipoprotein E Monoclonal Antibody, Unconjugated (bsm-61226R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with Apolipoprotein E monoclonal antibody, unconjugated (bsm-61226R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.