bsm-61239R [Primary Antibody]
SERCA1 Recombinant Antibody
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Host: Rabbit

Target Protein: SERCA1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 487

Swiss Prot: O14983

Source: KLH conjugated synthetic peptide derived from human SERCA1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Key regulator of striated muscle performance by acting as the major Ca2+ ATPase responsible for the reuptake of cytosolic Ca2+ into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen.Contributes to calcium sequestration involved in muscular excitation/contraction.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 110


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with SERCA1 monoclonal antibody, unconjugated (bsm-61239R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SERCA1 Monoclonal Antibody, Unconjugated (bsm-61239R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.