bsm-61303R [Primary Antibody]
INPP4B Recombinant Antibody
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Host: Rabbit

Target Protein: INPP4B

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 8821

Swiss Prot: O15327

Source: KLH conjugated synthetic peptide derived from human INPP4B

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-trisphosphate. Plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3,4-bisphosphate in membrane ruffles.The lipid phosphatase activity is critical for tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival

Size: 100µL

Concentration: Lot dependent

Applications: WB(WB(1:500-2000))
IHC-P(IHC-P(1:100-500))
IHC-F(IHC-F(1:100-500))
IF(IHC-P)(IF(IHC-P)(1:100-500))

Predicted Molecular Weight: 105


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with INPP4B monoclonal antibody, unconjugated (bsm-61303R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with INPP4B Monoclonal Antibody, Unconjugated (bsm-61303R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with INPP4B Monoclonal Antibody, Unconjugated (bsm-61303R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with INPP4B Monoclonal Antibody, Unconjugated (bsm-61303R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.