bsm-61331R [Primary Antibody]
CDC25C Recombinant Antibody
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Host: Rabbit

Target Protein: Cdc25C

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 995

Swiss Prot: P30307

Source: KLH conjugated synthetic peptide derived from human CDC25C

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle.When phosphorylated, highly effective in activating G2 cells into prophase.Directly dephosphorylates CDK1 and activates its kinase activity.

Size: 100µL

Concentration: Lot dependent

Applications: WB(WB(1:500-2000))
IF(ICC)(IF(ICC)(1:50-200))
IP(IP(1:20-50))

Predicted Molecular Weight: 53


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Cdc25C monoclonal antibody, unconjugated (bsm-61331R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


4% Paraformaldehyde-fixed K562 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CDC25C) monoclonal Antibody, unconjugated (bsm-61331R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.