bsm-61355R [Primary Antibody]
Phospho-CBL (Ser669) Recombinant Antibody
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Host: Rabbit

Target Protein: CBL (Ser669)

Modification Site: Ser669

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 867

Swiss Prot: P22681

Source: KLH conjugated synthetic peptide derived from human CBL around the phosphorylation site of S669

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 100


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Jurkat (H) cells were treated with or without pervanadate (1nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-CBL (Ser669) monoclonal antibody, unconjugated (bsm-61355R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.