bsm-61364R [Primary Antibody]
ACSS2 Recombinant Antibody
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Host: Rabbit

Target Protein: ACSS2

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 55902

Swiss Prot: Q9NR19

Source: KLH conjugated synthetic peptide derived from human ACSS2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids.Acetate is the preferred substrate.Can also utilize propionate with a much lower affinity,Nuclear ACSS2 promotes glucose deprivation-induced lysosomal biogenesis and autophagy, tumor cell survival and brain tumorigenesis.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 79


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with ACSS2 monoclonal antibody, unconjugated (bsm-61364R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ACSS2 antibody (bsm-61364R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.