bsm-61445R [Primary Antibody]
Integrin beta 4/CD104 Recombinant Antibody
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Host: Rabbit

Target Protein: Integrin beta 4/CD104

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 3691

Swiss Prot: P16144

Source: KLH conjugated synthetic peptide derived from human Integrin beta 4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling.

Size: 100µL

Concentration: Lot dependent

Applications: WB(WB(1:500-2000))
IHC-P(IHC-P(1:50-200))
IHC-F(IHC-F(1:50-200))
IF(IHC-P)(IF(IHC-P)(1:50-200))

Predicted Molecular Weight: 202


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Integrin beta 4/CD104 Monoclonal Antibody, Unconjugated (bsm-61445R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Integrin beta 4/CD104 Monoclonal Antibody, Unconjugated (bsm-61445R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Integrin beta 4/CD104 Monoclonal Antibody, Unconjugated (bsm-61445R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Integrin beta 4/CD104 Monoclonal Antibody, Unconjugated (bsm-61445R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with Integrin beta 4/CD104 monoclonal antibody, unconjugated (bsm-61445R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.