bsm-61454R [Primary Antibody]
Tyrosinase Recombinant Antibody
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Host: Rabbit

Target Protein: Tyrosinase

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 7299

Swiss Prot: P14679

Source: KLH conjugated synthetic peptide derived from human Tyrosinase

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone.

Size: 100µL

Concentration: Lot dependent

Applications: WB(WB(1:500-2000))

Predicted Molecular Weight: 60


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Tyrosinase monoclonal antibody, unconjugated (bsm-61454R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.