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Host: Rabbit
Target Protein: Ku70 / XRCC6
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 2547
Swiss Prot: P12956
Source: KLH conjugated synthetic peptide derived from human Ku70
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100µL
Concentration: Lot dependent
Predicted Molecular Weight: 70
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with Ku70 / XRCC6 monoclonal antibody, unconjugated (bsm-61460R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ku70 / XRCC6 Monoclonal Antibody, Unconjugated (bsm-61460R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ku70 / XRCC6 Monoclonal Antibody, Unconjugated (bsm-61460R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ku70 / XRCC6 Monoclonal Antibody, Unconjugated (bsm-61460R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ku70 / XRCC6 Monoclonal Antibody, Unconjugated (bsm-61460R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.