bsm-61479R [Primary Antibody]
Glucose 6 Phosphate Dehydrogenase Recombinant Antibody
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Host: Rabbit

Target Protein: Glucose 6 Phosphate Dehydrogenase

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 2539

Swiss Prot: P11413

Source: KLH conjugated synthetic peptide derived from human G6PD

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.

Size: 100µL

Concentration: 1mg/ml

Applications: WB(WB(1:500-2000))
FCM(FCM(1:50-100))
IHC-P(IHC-P(1:200-500))
IHC-F(IHC-F(1:200-500))
IF(IHC-P)(IF(IHC-P)(1:200-500))

Predicted Molecular Weight: 59


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Glucose 6 Phosphate Dehydrogenase Monoclonal Antibody, Unconjugated(bsm-61479R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Glucose 6 Phosphate Dehydrogenase Monoclonal Antibody, Unconjugated(bsm-61479R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Glucose 6 Phosphate Dehydrogenase Monoclonal Antibody, Unconjugated(bsm-61479R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Glucose 6 Phosphate Dehydrogenase Monoclonal Antibody, Unconjugated(bsm-61479R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucose 6 Phosphate Dehydrogenase Monoclonal Antibody, Unconjugated (bsm-61479R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with Glucose 6 Phosphate Dehydrogenase monoclonal antibody, unconjugated (bsm-61479R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


The 293T (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Glucose 6 Phosphate Dehydrogenase antibody (bsm-61479R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.