bsm-61491R [Primary Antibody]
WNT5A Recombinant Antibody
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Host: Rabbit

Target Protein: WNT5A

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 7474

Swiss Prot: P41221

Source: KLH conjugated synthetic peptide derived from human Wnt5a

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression.

Size: 100µL

Concentration: Lot dependent

Applications: WB(1:500-2000)
Flow-Cyt(1:50-100)
ICC/IF(1:50-200)

Predicted Molecular Weight: 42


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot analysis of (1) HeLa cell lysate; (2) SK-OV-3 cell lysate. Using WNT5A (bsm-61491R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.