DATASHEET
Host:
Rabbit
Target Protein:
gamma Sarcoglycan
Clonality:
Recombinant
Isotype:
IgG
Entrez Gene:
6445
Swiss Prot:
Q13326
Source:
KLH conjugated synthetic peptide derived from human gamma Sarcoglycan
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
VALIDATION IMAGES
Western blot analysis of Human skeletal muscle lysate. Using gamma Sarcoglycan (bsm-61504R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with gamma Sarcoglycan Monoclonal Antibody, Unconjugated (bsm-61504R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with gamma Sarcoglycan Monoclonal Antibody, Unconjugated (bsm-61504R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.