bsm-61545R [Primary Antibody]
CEACAM6 Recombinant Antibody
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Host: Rabbit

Target Protein: CEACAM6

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 4680

Swiss Prot: P40199

Source: KLH conjugated synthetic peptide derived from human CEACAM6

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cell surface glycoprotein that plays a role in cell adhesion and tumor progression.Intercellular adhesion occurs in a calcium- and fibronectin-independent manner.Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8.

Size: 100µL

Concentration: Lot dependent

Applications: WB(WB(1:500-2000))
IHC-P(IHC-P(1:100-500))
IHC-F(IHC-F(1:100-500))
IF(IHC-P)(IF(IHC-P)(1:100-500))
IF(ICC)(IF(ICC)(1:50-200))
IP(IP(1:20-50))

Predicted Molecular Weight: 37


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with CEACAM6 monoclonal antibody, unconjugated (bsm-61545R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human lung;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM6 Monoclonal Antibody, Unconjugated (bsm-61545R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human colon;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM6 Monoclonal Antibody, Unconjugated (bsm-61545R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human thyroid tumor;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM6 Monoclonal Antibody, Unconjugated (bsm-61545R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human colon cancer;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM6 Monoclonal Antibody, Unconjugated (bsm-61545R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.