bsm-61547R [Primary Antibody]
DHFR Recombinant Antibody
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Host: Rabbit

Target Protein: DHFR

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 1719

Swiss Prot: P00374

Source: KLH conjugated synthetic peptide derived from human Dihydrofolate reductase

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 21


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with DHFR monoclonal antibody, unconjugated (bsm-61547R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.