bsm-61559R [Primary Antibody]
RAD50 Recombinant Antibody
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Host: Rabbit

Target Protein: Rad50

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 10111

Swiss Prot: Q92878

Source: KLH conjugated synthetic peptide derived from human RAD50

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis.The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases.

Size: 100uL

Concentration: Lot dependent

Applications: WB(1:300-5000)
ICC/IF(1:50-100)

Predicted Molecular Weight: 154


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot analysis of Jurkat cell lysate; Using Rad50 (bsm-61559R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


4% Paraformaldehyde-fixed Jurkat (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RAD50) monoclonal Antibody, unconjugated (bsm-61559R) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.