bsm-61567R [Primary Antibody]
ROCK2 Recombinant Antibody
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Host: Rabbit

Target Protein: ROCK2

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 9475

Swiss Prot: O75116

Source: KLH conjugated synthetic peptide derived from human ROCK2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4.

Size: 100µL

Concentration: Lot dependent

Applications: WB(1:300-5000)
ICC/IF(1:50-100)

Predicted Molecular Weight: 161


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot analysis of (1) Hela cell lysate; (2) RAW264.7 cell lysate. Using ROCK2 (bsm-61567R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.