DATASHEET
Host:
Rabbit
Target Protein:
Alpha 1 microglobulin
Clonality:
Recombinant
Isotype:
IgG
Entrez Gene:
259
Swiss Prot:
P02760
Source:
KLH conjugated synthetic peptide derived from human AMBP
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Antioxidant and tissue repair protein with reductase, heme-binding and radical-scavenging activities. Removes and protects against harmful oxidants and repairs macromolecules in intravascular and extravascular spaces and in intracellular compartments.
VALIDATION IMAGES
25 ug total protein per lane of various lysates (see on figure) probed with Alpha 1 microglobulin monoclonal antibody, unconjugated (bsm-61579R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha 1 microglobulin Monoclonal Antibody, Unconjugated (bsm-61579R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.