bsm-61621R [Primary Antibody]
Human IgG3 Recombinant Antibody
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Host: Rabbit

Target Protein: Human IgG3

Clonality: Recombinant

Isotype: IgG

Swiss Prot: P01860

Source: KLH conjugated synthetic peptide derived from human Human IgG3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens

Size: 100uL

Concentration: Lot dependent

Applications: WB(1:500-2000)
IP(1:20-50)

Predicted Molecular Weight: 41


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Human IgG3 monoclonal antibody, unconjugated (bsm-61621R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.