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Host: Rabbit
Target Protein: IFIH1
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 64135
Swiss Prot: Q9BYX4
Source: A synthesized peptide derived from human MDA5
Purification: Purified by Protein A.
Storage Buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
WB(WB(1:500-2000))
IF(ICC)(IF(ICC)(1:50-200))
Predicted Molecular Weight: 117
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
THP-1 (H) cells were treated with or without LPS (1 μg/ml) for 24 h, 25 μg total protein per lane of cell lysates (see on figure) probed with IFIH1 monoclonal antibody, unconjugated (bsm-61660R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed THP-1 (treated with LPS (1 μg/ml, 24h)) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (IFIH1) monoclonal Antibody, unconjugated (bsm-61660R) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.