bsm-61675R [Primary Antibody]
TRIM25 Recombinant Antibody
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Host: Rabbit

Target Protein: TRIM25

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 7706

Swiss Prot: Q14258

Source: KLH conjugated synthetic peptide derived from human TRIM25

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection.

Size: 100µL

Concentration: Lot dependent

Applications: WB(1:500-2000)
IHC-P(1:50-200)
IHC-F(1:50-200)
IP(1:50-200)
IF
Flow-Cyt(1ug/Test)
ICC/IF(1:50-100)

Predicted Molecular Weight: 71


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Western blot analysis of HeLa cell lysate. Using TRIM25 (bsm-61675R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.