bsm-61688R [Primary Antibody]
ELAVL1 Recombinant Antibody
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Host: Rabbit

Target Protein: ELAVL1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 1994

Swiss Prot: Q15717

Source: KLH conjugated synthetic peptide derived from human ELAVL1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability.Involved in embryonic stem cell (ESC) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation (By similarity).

Size: 100µL

Concentration: Lot dependent

Predicted Molecular Weight: 36


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with ELAVL1 monoclonal antibody, unconjugated (bsm-61688R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Kidney Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Heart； Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Heart； Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Heart； Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer； Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer； Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.