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Host: Rabbit
Target Protein: RRM2
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 6241
Swiss Prot: P31350
Source: KLH conjugated synthetic peptide derived from human RRM2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100µL
Concentration: Lot dependent
Predicted Molecular Weight: 45
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with RRM2 monoclonal antibody, unconjugated (bsm-61703R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed PANC-1(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RRM2) monoclonal Antibody, unconjugated (bsm-61703R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.